3.4 PCR optimisation

Other variables that affect PCR are:

The best range for a particular set of primers for both these factors may be determined experimentally. In some cases the reaction may not work at all if the conditions are not in the correct range.

In some cases 5% DMSO (dimethyl sulphoxide) or 5% glycerol can be added to improve the efficiency of the PCR and increase the specificity of the primer binding. This does not work in all reactions and may sometimes inhibit the reaction altogether.

© SCBC 2007