List of Figures
Figure 5: How the Polymerase Chain Reacion Works
Figure 2: Denaturation of dsDNA
Figure 3: Annealing of primers to DNA template for PCR
Figure 4: Elongation from annealed primers in PCR
Figure 6: PCR of insulin-like growth factor
Figure 7: Hairpin loops and primer dimers
Figure 8: The vector is supplied in a linearised form ready for ligation to the insert
Figure 9: An EcoRI restriction site has been added so the PCR product can be digested and ligated to a cloning vector cut with the same enzyme.
Figure 1: A thermal cycler for PCR
Figure 10: Nested PCR using two sets of primer to reduce non-specific amplification of the gene of interest
Figure 11: Ligation Mediated PCR: An outline of the method
Figure 12: How Inverse PCR works
Figure 13: RT-PCR Synthesis of the first cDNA strand
Figure 14: The theory of assembly PCR
Figure 15: Quantitative PCR
Figure 16: RACE-PCR - Rapid amplification of cDNA ends
Figure 17: RACE-PCR
Figure 18: (1) Father. (2) Child. (3) Mother. The child has inherited some, but not all, of the fingerprint of each of its parents, giving it a new, unique fingerprint.
Figure 19: Cloning a gene using PCR.
Figure 20: DNA Shuffling: Random mutagenesis by PCR. The DNA is digested at random using DNaseI. The resulting fragments are denatured and used in PCR. At the annealing stage random complementary fragments bind.
Figure 21: Error prone polymerase mutagenesis. PCR is carried out using buffers which differ as shown.
Figure 22: Genotyping using PCR
Figure 23: Genotyping using PCR: table
© SCBC 2007